Spectral Characteristics: Visible absorption maxima=565>540>498 , Emission maximum=578; This goat anti-mouse IgG3 is used as the 'secondary antibody' for immunofluorescent staining on tissues or culture cells where the primary antibody is generated in mice. It is highly recommended that proper negative controls, such as omitting the primary antibody, be included in experiments when conducting immunofluorescent staining.