Gonadotropin-releasing Hormone (GnRH, LHRH, Luteinizing Hormorne Releasing Hormone, Progonadoliberin-1 precursor, Progonadoliberin I )

Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.\n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen): 1ug/ml. 4% paraformaldehyde is recommended as fixative\nImmunofluorescence: Staining of GnRH in mouse embryo brain tissue results in staining of neurons in the rostral forebrain.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMouse brain tissue sections\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8576-02

Size

100ul

Applications

IF, IHC

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Norwitz ER et al. Activin A augments GnRH-mediated transcriptional activation of the mouse GnRH receptor gene. Endocrinology 143:985-97 (2002). 2. Kennedy SR et al. Growth hormone response to a novel growth hormone-releasing tripeptide in horses: interaction with gonadotropin-releasing hormone, thyrotropin-releasing hormone, and sulpiride. J Anim Sci 80:744-50 (2002).

Additional Info

Recognizes human gonadotropin-releasing hormone. Species Crossreactivity: mouse. Species Sequence Homology: hamster, porcine, rat, Rhesus monkey, sheep, Xenopus laevis; 90%

Alternative Names

GnRH, LHRH, Luteinizing Hormorne Releasing Hormone,Progonadoliberin-1 precursor, Progonadoliberin I