GPR115 is a member of the LN-TM7 family of adhesion-type 7TM G-protein coupled receptors (GPCR) with long extracellular N-termini. The 385 aa N-terminal ECD contains a highly N-glycosylated stalk predicted to function in adhesion, followed by a cysteine-rich GPCR proteolytic cleavage site (GPS). Within the N-terminal region up to the GPS (aa22-347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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