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GPX1, Recombinant, Human, aa1-203, His-tag (Glutathione Peroxidase 1, GPx-1, GSHPx-1, Cellular Glutathione Peroxidase)

Cat no: G8127-11H

GPX1, Recombinant, Human, aa1-203, His-tag (Glutathione Peroxidase 1, GPx-1, GSHPx-1, Cellular Glutathione Peroxidase)

GPX1 (Glutathione peroxidase 1) belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. The GPX1 is part of the enzymatic antioxidant defence, preventing oxidative damage to DNA, proteins and lipids by detoxifying hydrogen and lipid peroxides that may contribute to prostate cancer development. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.\n\nSource:\nRecombinant corresponding to aa1-203 from human GPX1(U49C), fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLCG TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP SCA\n\nMolecular Weight:\n~24.2kD (MALDI-TOF)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8127-11H

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 2mM DTT, 30% glycerol, 100mM sodium chloride.

Purity

~90% (SDS-PAGE)

References

1. Ichimura Y., et al. (2004) J Urol.172 (2):728-32. 2. Li S, Yan T, et al. (2000) Cancer Res.Jul 60(14):3927-39.

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