Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G, Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1) (Biotin)

The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8750-01A5

Size

250ug

Applications

FC

Hosts

Rat

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. J Immunol.151:2399 - 2408.2. Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol.147:22 - 28.3. Czuprynski, C.J. et al. (1994) Administration of anti-Granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection. J Immunol.152:1836 - 1846.

Additional Info

Recognizes mouse Gr-1 antigen. Reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated.