Granzyme B (C11, Cathespin G Like 1, CCP1, CGL1, CSPB, CTLA1,CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, HLP, Human lymphocyte protein, Lymphocyte prot

Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes A to G, have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified. One of these, human granzyme B, is involved in target cell apoptosis during lymphocyte mediated cytotoxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell leads to the induction of DNA fragmentation and apoptosis.\n\nCellular Localization: Cytoplasmic granules of cytolytic T-Lymphocytes\n\nPositive Control: Suitable tissue control sections for Immunohistochemistry:Hodgkin?s lymphoma.\n\nApplications: \nSuitable for use in Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8957-03

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Tissue culture supernatant with sodium azide

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG2a

References

Andrade F et al. Granzyme B directly and efficiently cleaves several downstream caspase substrates: implications for CTL-induced apoptosis. Immunity 8:451-60 (1998). <PUBMED:9586635>Cho MS et al. Granzyme B immunoreactivity in T/natural killer cell lymphomas. Yonsei Med J 38:285-93 (1997). <PUBMED:9409191>de Bruin PC et al. Enteropathy-associated T-cell lymphomas have a cytotoxic T-cell phenotype. Histopathology 31:313-7 (1997). <PUBMED:9363445>

Additional Info

This antibody reacts with a 32kD protein. Species Crossreactivity: Crossreacts with Human.