Granzyme B (C11, Cathespin G Like 1, CCP1, CGL1, CSPB, CTLA1,CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, HLP, Human lymphocyte protein, Lymphocyte prot

Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. In the target cell, granzyme B rapidly induces apoptosis by activating both caspase-mediated as well as caspase-independent pathways. Apart from the storage granules of cytotoxic cells granzymes can also be detected in biological fluids such as serum and plasma. Elevated levels of soluble granzymes have been found in the sera of patients with increased cytotoxic responses, caused by various viral infections, as well as in the synovial fluid of rheumatic arthritis patients. A high percentage of GrB positive CTL has also been found in the glands of patients suffering from Hodgkin`s disease and in CTL infiltrates in transplant rejection.\n\nApplications:\nTested for the use in flow cytometry, ELISA and cell-ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8957-31

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC cells transiently transfected with a granzyme B expression vector.

Isotype

IgG1

Additional Info

Recognizes granzyme B transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells. Reacts with human granzyme B GrB), a 27kD serine protease which cleaves target-cell proteins at specific aspartate residues. Does not crossreact with the human granzymes A, K or M.