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Green Fluorescent Protein (Aequorea victoria) (GFP) (Texas Red)

Green Fluorescent Protein (Aequorea victoria) (GFP) (Texas Red)

Cat no: G8965-14A

Supplier: United States Biological
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Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8965-14A
Hosts: Goat
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 200ug
Form: Supplied as a liquid in PBS, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, pH7.2 Labeled with Texas Red (A=589, E=615).
P type: Pab
Isotype: IgG
Purity: Purified
Alternative names: Green Fluorescent Protein
References: Titus JA et al. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies. J Immunol Methods 50:193-204 (1982).
Additional info: Recognizes wild type, recombinant and enhanced forms of GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against human, mouse or rat serum proteins.

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