Green Fluorescent Protein (GFP) (PE)

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Our anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Our anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry.\n\nRecommended Dilution:\nELISA: 1:3,000\nWestern Blot: 1:2,000\nImmunohistochemistry: 1:50~100\nImmuno-fluorescence: 1:50~100\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store . Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze R-Phycoerythrin conjugates. Light sensitive.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts