GFP [HRP] Monoclonal Antibody is highly immunoaffinity purified from mice ascites by protein A and then conjugated to horseradish peroxidase. GFP [HRP] can be used to detect various forms of GFP proteins in western blot analyses.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.02-0.5ug/ml
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.