Griffonia (Bandeiraea) simplicifolia GS II

Supplier:

United States Biological

Cat no: G8967-80

Griffonia (Bandeiraea) simplicifolia GS II

The seeds of Griffonia simlicifolia conain four distinct lectins, 30,000D each. Two of these lectins, referred to as GS-I and GS-II have been well characterized with regard to binding specificity and structure. GS-II is specific for terminal, non-reducing alpha or beta linked N-acetyl-D-glucosamine. This lectin is not blood group specific, unlike GS-I. GS-II is also a tetramer but it is composed of identical subunits. Each subunit has a single carbohydrate binding site for GlcNAc. GS-II is the only lectin isolated that is specific for only a terminal GIcNAc residue. The subterminal saccharide does play an important role in lectin binding. \n\nInhibiting/Eluting Sugar: Chitin Hydrolysate or 200mM N-acetylglucosamine\n\nActivity: 5-10ug/ml will agglutinate Tk polyagglutinaable cells.\n\nInhibitory Carbohydrate: N-acetylglucosamine\n\nCarbohydrate Specificity: Terminal a- or b- N- Acetylglucosamine. The specific linkage of N-Acetylglucosamine to the subterminal carbohydrate plays an important role in lectin binding.\nNote: Calcium is required for binding. 0.5mM calcium is the maximum concentration in buffer that will not form a white precipitate.\n\n\n

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SPECIFICATIONS

Catalog Number

G8967-80

Size

1mg

Applications

IHC

Alternative Names

Bandeiraea simplicifolia

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

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