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Growth Hormone, Porcine, Recombinant

Cat no: G8999-07

Growth Hormone, Porcine, Recombinant

Recombinant porcine Growth Hormone is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21.73kD. \n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Phe-Pro-Ala-Met-Pro.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nBiological Activity:\n3units/mg\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n 1. UV spectroscopy at 280nm using the absorbancy value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n 2. Analysis by RP-HPLC, using a calibrated solution of GH-20K as a Reference Standard. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 6 months at -20 degrees C. Reconstitute with sterile ddH2O to a concentration (same/more than) 0.1mg/ml. for long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot and store at -20 degrees C. Reconstituted product is stable for 1 month at 4 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8999-07

Size

100ug

Form

Supplied as a powder lyophilized from a solution containing PBS. Reconstitute with sterile ddH2O to a concentration (same/more than) 0.1mg/ml.

Purity

(same/more than) 98% by RP-HPLC and SDS-PAGE. Chromatographically purified.

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