GRP94 Monoclonal Antibody (Clone 9G10)

Antigen: purified recombinant GPR94 protein . Host: rat, clone 9G10 . Cross Reactivity: (+) human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, horse, monkey, porcine, rabbit, ovine, and Xenopus GRP94 . Applications: WB. IP, and flow cytometry . Isotype: IgG2a

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SPECIFICATIONS

Catalog Number

10011424

Applications

IP, WB

Hosts

Rat

Form

200 microg

P Type

Antibodies

Weight

98

Antigen

purified recombinant GPR94 protein

Isotype

IgG2a

Storage Temp

-20

Shipping Temp

-20

Additional Info

Glucose regulated protein 94 (GRP94) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. GRP94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion. GRP94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific cytotoxic T lymphocyte (CTL) responses to chaperone bound peptides via the major histocompatibility complex (MHC) class I pathway. GRP94 is a member of the Hsp90 family of stress proteins and shares sequence homology with its cytosolic equivalent, Hsp90. Both Hsp90 and GRP94 are calcium binding proteins. Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, GRP94, and Hsp90 differ in their interactions with regulatory ligands as GRP94 has weak ATP binding and hydrolysis activity. GRP94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C-terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. GRP94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER.