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GSTO1, 140 D, Recombinant, Human (GSTTLp28, P28, glutathione S-transferase omega 1-1, glutathione transferase omega, glutathione transferase omega 1, glutathione-S-transferase like, Monomethyl\nArsenous Reductase)

Cat no: G8135-21J

GSTO1, 140 D, Recombinant, Human (GSTTLp28, P28, glutathione S-transferase omega 1-1, glutathione transferase omega, glutathione transferase omega 1, glutathione-S-transferase like, Monomethyl\nArsenous Reductase)

GSTO1 is a member of the theta class glutathione S-transferase-like (GSTTL) protein family. GSTO1 is expressed in a wide range of human tissues and exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities and also catalyzes the reduction of monomethylarsonate, an intermediate in the pathway of arsenic biotransformation. GSTO1 protects from oxidative stress, a risk factor for Alzheimer disease, vascular dementia and stroke. Several polymorphisms in the coding regions of the human GSTO1 have been identified. A polymorphism causing an alanine-to-aspartate (A140D) substitution in aa140 produces a variant with lowered enzyme activities in the arsenic biotransformation.\n\nSource: \nRecombinant corresponding to aa1-241 from human 140D mutant mature GSTO1, fused to 6x His-tag at N-terminal, expressed in E. coli.\n\nApplications:\nSuitable for use in Western Blot as a control. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8135-21J

Size

10ug

Applications

WB

Form

Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Purity

Purified (SDS-PAGE)

References

1. Withbread, A. K. et al., Methods in Enzymology 2005; 401: 78-99. 2. K

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