HCLS1 (HCLS1, HS1, hematopoietic cell-specific Lyn substrate 1),Human

HS1, also called hematopoietic cell-specific LYN substrate 1 (HCLS1), LckBP1, p75, and hematopoietic lineage cell-specific protein, is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains 4 cortactin repeats and one SH3 domain (2). It is phosphorylated after activation of immune receptors (3-5), leading to its recruitment to the immune synapse. Phosphorylation of HS1 is also required for regulating actin dynamics and provides docking sites for many other signaling molecules, such as Vav1 and PLCg1 (6). HS1 also plays an important role in platelet activation (7).\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern blot: 1:1000\nImmunoprecipitation: 1:50\nFlow Cytometry: 1:25\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1827-01F

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

(1) Kitamura, D. et al. (1989) Nucleic Acids Res 17,9367

Additional Info

Detects endogenous levels of total HS1 protein. This antibody does not recognize mouse and rat HS1 proteins. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels.