HCV, NS5B (Nonstructural Protein 5B)

The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.\n\nApplications:\nSuitable for use in Western Blot\n\nRecommended Dilution:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

044882

Size

100ug

Applications

WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002)\n2. Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells: R.W. Hardy, et al.; J. Virol. 77, 2029 (2003)\n3. Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs: G. Randall, et al.; PNAS 100, 235 (2003)\n4. Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005)\n5. Quantitative analysis of the hepatitis C virus replication complex: D. Quinkert, et al.; J. Virol. 79, 13594 (2005)\n6. Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3: Y.V. Svitkin, et al.; J. Virol. 79, 6868

Additional Info

Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). Recognizes genotype 1a and also 1b (albeit with lower affinity). Does not recognize genotype 2a.

Alternative Names

Nonstructural Protein 5B