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HDAC11, Recombinant, Human, GST-Tag (Histone Deacetylase 11)

Cat no: H1827-57A

HDAC11, Recombinant, Human, GST-Tag (Histone Deacetylase 11)

Human HDAC11 (full length) (GenBank Accession No. BC009676) with N-terminal GST tag, expressed in baculovirus expression system. \n\nSpecific Activity: \n~1 pmol/min/ug\n\nAssay Conditions: \n25mM Tris/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2 and 0.1mg/ml BSA, 20uM BPS HDAC substrate and 40ng/ul HDAC11. \n\nIncubation Condition: \n60 min at 37C. \n\nApplications: \nSuitable for the use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

H1827-57A

Size

50ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20 and 50% glycerol.

Purity

~80%

References

1. Strausberg,R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

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