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HE+ Pervanadate Lysate

Cat no: H1833A

HE+ Pervanadate Lysate

Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. Human aortic endothelial cells treated with pervanadate for 30 minutes display a significant increase in tyrosine phosphorylation as shown by western\nblotting using anti-Phosphotyrosine. Confluent cultures of human aortic endothelial cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1 mM) for 30 minutes at 37 degrees C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate,\n10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.\n\nApplications:\nSDS-PAGE/Western blotting 20ml/well.\nEnd user should determine optimal quantity for their particular applications and experiments.

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SPECIFICATIONS

Catalog Number

H1833A

Size

100ug

Applications

WB

Form

Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).

References

1981 Ross, A.H. et al. Nature 294:654.\n1988 Wang, J.Y.J. Anal. Biochem 172:1.

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