Heat Shock Cognate Protein 70 (HSC70, HSP73)

The HSP 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP 70 family members include: HSP 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP 72, a 72kD protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72kD, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP 78, or BiP, is a glucose regulated 78kD protein localized in the endoplasmic reticulum; and p75, or Hsc 75, a 75kD protein that is found within the mitochondria. Hsc 70 (also known as Hsc 71, Hsc 73, Hsc 73, p72, prp 73) is expressed constitutively and is slightly heat-inducible. Hsc 70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. Hsc 70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.\n\nApplications: \nSuitable for use in Western Blo, Immunoprecipitation and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1831-25J

Size

50ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

This product has been used in:Maekawa M et al. Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells. Biol Reprod 40:843-52 (1989). <PUBMED:2665832> Leung SM & Hightower LE A 16-kDa protein functions as a new regulatory protein for Hsc70 molecular chaperone and is identified as a member of the Nm23/nucleoside diphosphate kinase family. J Biol Chem 272:2607-14 (1997). <PUBMED:9006893>Terlecky SR et al. Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein. J Biol Chem 267:9202-9 (1992). <PUBMED:1577755>Gething MJ & Sambrook J Protein folding in the cell. Nature 355:33-45 (1992). <PUBMED:1731198>Allen RL et al. A novel hsp70-like protein (P70) is present in mouse spermatogenic cells. Mol Cell Biol 8:828-32 (1988). <PUBMED:3352605>

Additional Info

This antibody displays slight crossreactivity to HSP 70. Species Crossreactivity: Crossreacts with Human and Mouse.