Heat Shock Cognate Protein 70 (HSC70, HSP73)

HSC70-interacting protein (Hip) is a cytosolic protein that exists as a homo-oligomer and participates in the regulation of the heat shock protein HSC70 in eukaryotic cells. Hip interacts with the ATPase domain of HSC70 molecules through the tetratricopeptide repeats and flanking charged a-helices of Hip. This binding reaction is dependent on HSP40 in the presence of ATP and becomes Hsp40-independent if HSC70 is preincubated with ADP. Upon binding to HsSC70, Hip stabilizes the ADP-bound state of HsSC70, a conformation that has a high affinity for unfolded substrate proteins. Hip by itself binds unfolded polypeptides, suggesting that it may possess chaperone activity. Together with HSP40, Hip may regulate the chaperone activity of HSC70 by stabilizing the chaperone- substrate complex. \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2500\nImmunohistochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1831-25M

Size

50ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Fel, NHP

Form

Supplied as a liquid in PBS, 0.05% sodium azide before addition of glycerol to 40%.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells: M. Maekawa, et al.; Biol. Reprod. 40, 843 (1989)

Additional Info

Recognizes mouse HSC70 (heat shock cognate protein 70) at ~70kD by Western Blot. Species Crossreactivity: human, monkey and feline. Crossreactivity: Slightly with HSP70.