Heat Shock Protein 101 (Hsp101/ClpB- C-terminal)

Hsps/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (2, 3). Hsp100 is one such major class that acts as a chaperone. Hsp101/ClpB is a member of this Hsp100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be \nrefolded by other chaperones. In spite of expression during heat stress, members of Hsp100 protein family are also expressed during seed development. Hsp101 protein is both nuclear and cytoplasmic-localized (1, 4). \n \nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1832-01C

Size

200ul

Applications

WB

Hosts

Rabbit

Form

Supplied as a lyophilized powder. Reconstitute with 200ul of sterile water.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Nieto-Sotelo J., et al. (2002) Plant Cell. 14:1621-1633. 2. Vierling E. (1991) Annu Rev. Plabt Physiol. Plant Mol. Biol. 42:579-620. 3. Waters W., et al. (2004) Trends Plant Sci. 9: 244-252. 4. Nieto-Sotelo J., Kannan K.B., Martinex L.M. and Segal C. (1999) Gene. 230:187-195.

Additional Info

Recognizes hsp101. Species Crossreactivity: Zea mays. No reactivity to other cereal Hsp101.