Heat Shock Protein 22 (HSP22, H11, E2IG1, HSPB8, Heat shock protein beta-8, E2-induced gene 1, spinal muscular atrophy, distal, adult, autosomal dominant, small stress protein-like protein HSP22, protein kinase H11, hereditary motor neuropathy, distal, he

HSPB8 (heat shock protein beta-8), otherwise known as HSP22, a monomeric protein and member of the small heat shock protein (HSP20) family, which displays temperature dependent chaperone activity. HSPB8 is predominantly expressed in the heart and skeletal muscle, and defects in HSPB8 are responsible for the neuromuscular disorder distal hereditary motor neuronopathy type 2A (HMN2A), and the axonal CMT neuropathy Charcot Marie Tooth disease type 2L (CMT2L).\n\nApplication(s):\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: ~20-25kD band seen in Human Muscle lysate [Predicted MW of ~22kD according to NP_055180]. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Muscle lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-48M

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

General/background: Benndorf R et al. HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27). J Biol Chem 276:26753-61 (2001).

Additional Info

Recognizes human Heat Shock Protein 22.