Heat Shock Protein 22, Recombinant, Human (Heat Shock 22kDa Protein 8, HSP22, Heat Shock Protein beta-8, HSPB8, alpha-Crystallin C Chain, CRYAC, CMT2L, DHMN2, H11, HMN2, HMN2A, E2-induced Gene 1 Protein, E2IG1, PP1629, Protein Kinase H11, Small Stress Pro

Supplier:

United States Biological

Cat no: H1830-48T

Heat Shock Protein 22, Recombinant, Human (Heat Shock 22kDa Protein 8, HSP22, Heat Shock Protein beta-8, HSPB8, alpha-Crystallin C Chain, CRYAC, CMT2L, DHMN2, H11, HMN2, HMN2A, E2-induced Gene 1 Protein, E2IG1, PP1629, Protein Kinase H11, Small Stress Pro

Heat shock protein beta-8 (HSPB8), also known as HSP22, member of the small heat shock protein superfamily. HSPB8 is predominantly transcribed in skeletal muscle and heart. The chaperone-like activity is of great importance to the function of HSPB8 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L). Recombinant human HSPB8, fused to His-tag at N-terminus, was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa1-196 of human HSPB8/HSP22 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MADGQMPFSC HYPSRLRRDP FRDSPLSSRL LDDGFGMDPF PDDLTASWPD WALPRLSSAW PGTLRSGMVP RGPTATARFG VPAEGRTPPP FPGEPWKVCV NVHSFKPEEL MVKTKDGYVE VSGKHEEKQQ EGGIVSKNFT KKIQLPAEVD PVTVFASLSP \nEGLLIIEAPQ VPPYSTFGES SFNNELPQDS QEVTCT\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-48T

Size

50ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl pH 8.0, 100mM NaCl, 10% glycerol.

Purity

~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Sun X., et al. (2004) J Biol Chem. 279(4):2394-402. 2. Benndorf R., et al. (2001) J Biol Chem. 276:26753-61.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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