Heat Shock Protein 27 (Heat Shock 27 kD Protein, HSP 27, HSP27, 28 kD Heat Shock Protein, CMT2F, Estrogen-regulated 24 kD Protein, Heat Shock Protein beta-1, HspB1, HMN2B, Hsp25, HSP28, Stress-responsive Protein 27, SRP27)

Human Hsp27 belongs to the phylogenically conserved small heat shock protein (smHsp) family, which includes mouse Hsp25, alphaA-crystallins and alphaB-crystallins. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. It is believed to exist mainly as oligomers of as many as 8-40 of Hsp27 protein monomers in cells and data suggest that these large oligomers have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is phosphorylated on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2/3 and the stress-activated protein kinase SAPK2 (p38).\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cells\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-50L

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Mehlen, P. et al. Biochem. Biophys. Res. Commun. 241:187-192 (1997). 2. Benndorf, R. et al. J. Biol. Chem. 269:20780-20784 (1994). 3. Butt, E. et al. J. Biol. Chem. 276:7108-7113 (2001).

Additional Info

Recognizes human HSP27.

Alternative Names

Heat Shock 27kDa Protein 1, DKFZp586P1322, HS.76067