Heat Shock Protein 27 (HSP27) BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human HSP27 in cell lysates.\n\nHsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, a-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kD.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP04792\n\nEntrez Gene ID:\n3315\n\nKit Components:\n96-well plate pre-coated with anti-HSP27, Human\nLyophilized recombinant HSP27, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143862

Size

96Tests

References

1. Hickey, E., Brandon, S. E., Potter, R., Stein, G., Stein, J., Weber, L. A. Sequence and organization of genes encoding the human 27 kDa heat shock protein. Nucleic Acids Res. 14: 4127-4145, 1986. (Note: Erratum: Nucleic Acids Res. 14: 8230 only, 1986.)\n2. Hunt, C. R., Goswami, P. C., Kozak, C. A. Assignment of the mouse Hsp25 and Hsp105 genes to the distal region of chromosome 5 by linkage analysis. Genomics 45: 462-463, 1997.\n