Heat Shock Protein 27, Recombinant, Human (HSP27)

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant HSP-27 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 205 amino acids and having a molecular mass of 22.7kD. Recombinant Human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.\n\nImmunological Activity: \nImmunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSP25/27.\n\nSequence: \nMTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK\n\nStoage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-51A1

Size

10ug

Form

Supplied as a liquid in 20mM HEPES, pH 7.5, 1mM DTT and 100mM potassium chloride.

Purity

(same/more than) 95% as determined by RP-HPLC and SDS-PAGE Coomassie staining.

References

1. Heat Shock Protects Cardiac Cells From Doxorubicin-Induced Toxicity By Activating p38MAPK and Phosphorylation of Small Heat Shock Protein 27. Am J Physiol Heart Circ Physiol 2006 Jun 16; 2. Overexpression of heat shock protein 27 is associated with good prognosis in the patient with oral squamous cell carcinoma. Br J Oral Maxillofac Surg 2006 May 23; 3. Expression of heat shock protein 70 and 27 in non-small cell lung cancer and its clinical significance. Huazhong Univ Sci Technolog Med Sci 2005;25(6):693-5 4. Ischemia-specific phosphorylation and myofilament translocation of heat shock protein 27 precedes alpha B-crystallin and occurs independently of reactive oxygen species in rabbit myocardium. J Mol Cell Cardiol 2006 Jun;40(6):761-74 5. [Regulation of heat shock protein 27/activating transcription factor-5 complex and its implication in podocyte apoptosis induced by high glucose] Zhonghua Yi Xue Za Zhi 2006 Feb 14;86(6):394-8 6. Effect of exercise on activation of the p38 mitogen-activated protein kinase pathway, c-Jun NH2 terminal kinase, and heat shock protein 27 in equine skeletal muscle. Am J Vet Res 2006 May;67(5):837-44