Heat Shock Protein 27, Recombinant, Human (HSP27)

Hsp27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an a-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The upregulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (6).\nApplications:\nSuitable for use as a Western Blot control, in Binding Assays and as an ELISA reference standard. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-51H

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in 20mM phosphate buffer, 150mM sodium chloride and 10% glycerol.

Purity

(same/more than) 90% pure as determined by SDS-PAGE analysis.

References

1. Kim, K.K., Kim, R., and Kim, S., Nature 394(6693): 595-599 (1998). 2. Van Montfort, R., Slingsby, C., and Vierling, E., Addv. Protein Chem. 59: 105-156 (2001). 3. Ehrnsperger, M., Graber, S., Gaestel, M., and Buchner, J., EMBO J. 16: 221-229 (1997). 4. Ciocca, D.R., Oesterreich, S., Chamness, G.C., McGuire, W.L., and Fugua, S.A., J. Natl. Cancer Inst. 85(19): 1558-1570 (1993). 5. Sarto, C., Binnz, P.A., and Mocarelli, P., Electrophoresis. 21(6): 1218-1226 (2000). 6. Arrigo, A.P., J. Cell Biochem. 94(2): 241-246 (2005).