Heat Shock Protein 27, Recombinant, Human (HSP27)

Supplier:

United States Biological

Cat no: H1830-57H

Heat Shock Protein 27, Recombinant, Human (HSP27)

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and consists of 205 amino acids.\n\nSequence:\nMTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-57H

Size

100ug

Form

Supplied as a liquid in 20mM HEPES, pH 7.5, 1mM DTT and 100mM potassium chloride.

Purity

~95% by SDS-PAGE. Purified by using conventional chromatography techniques.

References

1. Ehrnsperger, M., et al., EMBO J. 16: 221-229 (1997). 2. Butt, E., et al., J. Biol. Chem. 276: 7108-7113 (2001).

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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