Heat Shock Protein 47 (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antigen RA-A47, SERPINH2)

HSP47, also known as heat shock protein 47, colligin, or gp46, is a collagen-specific stress glycoprotein which resides in the ER and has been shown to be involved in the synthesis/assembly of various collagens as a molecular chaperone. The protein belongs to the serpin (serine protease inhibitor) superfamily but unlike other serpin family proteins, it localizes within the endoplasmic reticulum (ER) because of the presence of RDEL (Arg-Asp-Glu-Leu) sequence, a variant of KDEL sequence, at the C-terminus. HSP47 has been shown to bind to the KDEL receptor in the ER. HSP47 is susceptible to phosphorylation. Both gp46 and HSP47 were later found to be heat-inducible and susceptible to phosphorylation. It is generally believed that HSP47 binds procollagen immediately after it co-translationally enters the ER, and it dissociates from procollagen before its secretion. When cells are heat shocked or treated with inhibitors of the triple-helix formation of procollagen, HSP47 is retained to these abnormal or denatured procollagens for much longer periods of time. Expression of HSP47 on cell surface following alkalization of the endosomal compartments under conditions of stress suggest HSP47 may be a serpin family protein that may modulate cell migration during development and invasion and metastasis in cancer. HSP47 has been suggested to play an important role in tumor metastasis and can be used as a prognostic marker for the metastatic activity of human tumor cells.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 using ECL.\nImmunohistochemistry: 1:50\nOptimal dilutions to be determined by researcher.\n \nRecommended Positive Controls:\nHeLa Heat Shocked Cell Lysate\nRecombinant Rat HSP47 Protein\nHuman Breast Cancer Tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1834-20

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Cates, G.A., et al., Biochem. Cell Biol. 65: 767-775 (1987). 2. Nagata, K. and Yamada, K.M., J. Biol. Chem. 261: 7531-7536 (1986). 3. Razzaque, M.S. and Taguchi, T., Kidney Int. Suppl. 71: S256-S259 (1999). 4. Kuroda, K., et al., J. Invest. Dermatol. 111: 1023-1028 (1998). 5. Jain, N., et al., Arch. Biochem. Biophys. 314: 23-30 (1994). 6. Lopes, M.A., et al., J. Oral Maxillofac. Surg. 60(2): 142-147 (2002). 7. Loones, et al., Cell Stress & Chaperones 3: 237-244 (1998). 8. Sauk, J.J. et al. (1998) Connective Tissue Rsch. 37, 105-119. 9. Dafforn, T.R., et al., J. Biol. Chem. 276(52): 49,310-49,319 (2001).

Additional Info

Recognizes rat HSP47 at ~47kD. Species Crossreactivity: human, monkey, mouse, rabbit, hamster, guinea pig, bovine, canine, sheep, porcine and chicken