Heat Shock Protein 60 (60kD Heat Shock Protein, Mitochondrial, 60kD Chaperonin, Chaperonin 60, CPN60, Heat Shock Protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein, HSPD1)

HSP60, a 60kD member of Chaperonin family, is a mitochondrial protein that participates in protein folding, transport and secretion. HSP60 is a multi-functional protein whose roles include accelerating the maturation of pro-caspase 3 during apoptosis, LPS-binding, acting as a ligand for innate immune system and also binding to the surface of innate immune cells and stimulating proinflammatory reactivity. It induces the expression of IL-12 and IL-15 and also induces release of TNF-alpha, NO and IL-6. Reports suggest interaction of Hsp60 with integrin molecules during metastasis. Mutations in Hsp60 gene cause autosomal recessive spastic paraplegia 13. Hsp60 has been found to be involved in development of auto-immune disorders such as Rheumatoid arthritis, insulin-dependent diabetes mellitus, systemic sclerosis and atherosclerosis.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.2-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa, Jurkat cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1834-25S

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Bov, Can, Ch/Bird, NHP

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Satoh,J., Onoue,H., Arima,K. and Yamamura,T. The 14-3-3 protein forms a molecular complex with heat shock protein Hsp60 and cellular prion protein. J. Neuropathol. Exp. Neurol. 64 (10), 858-868 (2005) 2. Jamin,C., Dugue,C., Alard,J.E., Jousse,S., Saraux,A., Guillevin,L., Piette,J.C. and Youinou,P. Induction of endothelial cell apoptosis by the binding of anti-endothelial cell antibodies to Hsp60 in vasculitis-associated systemic autoimmune diseases. Arthritis Rheum. 52 (12), 4028-4038 (2005) 3. Cohen-Sfady,M., Nussbaum,G., Pevsner-Fischer,M., Mor,F., Carmi,P., Zanin-Zhorov,A., Lider,O. and Cohen,I.R. Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway. J. Immunol. 175 (6), 3594-3602 (2005)

Additional Info

Recognizes human Chaperonin/Hsp60. Species Crossreactivity: bovine, broad, chicken, chimpanzee, canine, monkey.