Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

Hsp60 is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60. Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES. Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 0.25ug/ml, 10ug of heat shocked HeLa cell lysate.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-70C

Size

50ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Prc, Rabbit, Yeast/Fun

Form

Supplied as a liquid in PBS, 0.1mM PMSF, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Hartl F.U. (1996) Nature 381: 571-579. 2. Bukau B. and Horwich A.L. (1998) Cell 92: 351-366. 3. Hartl F.U. and Hayer-Hartl M. (2002) Science 295:1852-1858. 4. Jindal S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda D., et al. (1999) Infect Dis. Obstet. Gynecol. 7:64- 71. 6. Itoh H. et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta S. and Knowlton A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris C.C. et al. (2006) Cell Stress Chaperones 11: 116- 128. 9. Lai H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297. 10. Gao Y.L., et al (1995) J. of Immunology 154: 3548-3556. 11. Neuer A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929. 12. Bason C., et al (2003) Lancet 362(9400): 1971-1977.

Additional Info

Recognizes human HSP60 at ~60kD. Epitope: aa383-447. Species Crossreactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit, spinach, E. coli (GroEl), H. pylori, S. typhimurium, T. spiralis, yeast, white fly.