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Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

Cat no: H1830-70J

Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1000-1:3,000\nWestern Blot: 1:1000-1:3,000\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nMouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP60 protein. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-70J

Size

50ul

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

Cheng MY, et al., (1990) Nature. 348:455-8\nGhosh JC, et al., (2008) J Biol Chem. 283(8):5188-94

Additional Info

Recognizes human Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60).

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