Heat Shock Protein 70 (Heat Shock 70 kD Protein, HSP70, b0014, Chaperone Protein DnaK, dnaK, groP, grpF, JW0013, Molecular Chaperone DnaK, seg)

The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed proteins. All of the Hsp70 proteins have three major functional domains. An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate), a substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues and a C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. Eukaryotic organisms express several slightly different Hsp70 proteins. Hsc70 or Hsp73; a constitutively expressed chaperone protein, Hsp70 or Hsp72; a stress-induced protein, BiP or Grp78; a protein localized to the endoplasmic reticulum mtHsp70 or Grp75; the mitochondrial Hsp70. Heat shock protein 72 (Hsp72) is the major inducible, 70kD heat shock protein. It plays a role in many cellular activities including protein synthesis, folding, and translocation into organelles as well as the assembly of multiprotein complexes. Hsc70 typically makes up one to three percent of total cellular protein. The genes are located in the major histocompatibility complex class III region.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:75-1:250\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBreast carcinoma\n\nCellular Localization: \nCytoplasmic and nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1834-35D

Size

500ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Rothman J E Cell 59: 591, 1989. 2. Flaherty et al. Nature 346: 623, 1990. 3. Wegele et al. Rev Physiol Biochem Pharmacol 151:1, 2004. 4. Yoshimura et al. Biochem Biophys Res Commun 293: 1389, 2002.

Additional Info

Recognizes human Hsp70.