Heat Shock Protein 70, Mitochondrial (HSPA9B, mtHSP70, GRP75, Mortalin 2)

The HSP 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP 70 family members include: HSP 70, a 70kD protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP 72, a 72kD protein which is induced exclusively under stress conditions; HSC 70, or cognate protein, a 72kD constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP 78, or BiP, a glucose-regulated 78kD protein localized to the endoplasmic reticulum; and mitochondrial HSP 70 (mtHSP 70, GRP 75 or mortalin), a 75kD protein that is found within the mitochondria. mtHSP 70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP 70 and other inner membrane proteins of the mitochondria. \n\nApplication(s): Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation.\n\nRecommended Dilution:\nImmunocytochemistry: 1:50\nWestern Blot: 1:500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

H1830-91A

Size

100ul

Applications

ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

sSupplied as liquid ascites in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG3

References

1. Generation and characterization of monoclonal antibodies specific for members of the mammalian 70-kDa heat shock protein family: J.M. Green, et al.; Hybridoma 14, 347 (1995) 2. Cytochrome c oxidase subassemblies in fibroblast cultures from patients carrying mutations in COX10, SCO1 or SURF1: S.L. Williams, et al.; J. Biol. Chem. 279, 7462 (2004) 3. The life span determinant p66Shc localizes to mitochondria where it associates with mitochondrial heat shock protein 70 and regulates trans-membrane potential: F. Orsini, et al.; J. Biol. Chem. 279, 25689 (2004)

Additional Info

Recognizes human and mouse mtHSP70. Detects a band of ~75kD by Western blot. Species Crossreactivity: Human, mouse.\n