Heat Shock Protein 70, Recombinant, Mycobacterium tuberculosis (HSP70)

Dimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of MTHSP70.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a calibrated solution of Human Heat Shock Protein as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized MTHSP70 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1830-92M

Size

2ug

Form

Supplied as a lyophilized powder in 10mM sodium phosphate buffer, pH 7.4, 130mM sodium chloride, 2.5mM potassium chloride.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. [Cloning and prokaryotic expression of heat shock protein 70 gene of Mycobacterium tuberculosis] Ye J, Sui YF, Zhang XM, Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2003 Sep;19(5):443-5 2. The adjuvant effects of Mycobacterium tuberculosis heat shock protein 70 result from the rapid and prolonged activation of antigen-specific CD8+ T cells in vivo. Harmala LA, Ingulli EG, Lucido MM, Mescher MF, J Immunol 2002 Nov 15;169(10):5622-9 3. Enhancement of Sindbis virus self-replicating RNA vaccine potency by linkage of Mycobacterium tuberculosis heat shock protein 70 gene to an antigen gene. Cheng WF, Hung CF, Hsu KF, Wu TC, J Immunol 2001 May 15;166(10):6218-26 4. Enhancement of suicidal DNA vaccine potency by linking Mycobacterium tuberculosis heat shock protein 70 to an antigen. Hsu KF, Hung CF, He L, Gene Ther 2001 Mar;8(5):376-83 5. Polymorphism in heat-shock protein 70-1 (HSP70-1) gene promoter region and susceptibility to tuberculoid leprosy and pulmonary tuberculosis in Asian Indians. Rajalingam R, Mehra NK, Indian J Exp Biol 2000 Jul;38(7):658-62 6. Expression of foreign gene in mycobacterium regulated by human Mycobacterium tuberculosis heat shock protein 70 promoter. Cheng J, Huangfu Y, Liang J, J Tongji Med Univ 1997;17(4):193-9.