Heat Shock Protein 90, Beta, BioAssay(TM) Kit (Heat Shock Protein HSP 90-beta, HSP90b, HSP 90, D6S182, FLJ26984, Heat Shock 84kD, HSP 84, HSP84, Heat Shock Protein 90kDa alpha (Cytosolic) Class B Member 1, HSP90AB1, HSPC2, HSPCB)

Hsp90bis a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90b functions has been shown to play a role in tumorigenesis and disease progression. The Hsp90b Assay Kit is designed for identification of Hsp90b inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin, an HSP90 inhibitor, for binding to purified recombinant Hsp90b.\n\nIntended Use:\nDesigned for identification of Hsp90b inhibitors using fluorescence polarization.\n\nTest Principle:\nThe Hsp90b inhibitor screening assay kit comes in a convenient 96-well or 384-well format, with purified Hsp90b enzyme, FITC-labeled geldanamycin, and Hsp90 assay buffer for 100 enzyme reactions. The key to the Hsp90b Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for Hsp90b reactions. The FITC-labeled geldanamycin is incubated with a sample containing Hsp90b enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.\n\nKit Components:\nHsp90b recombinant enzyme: 1x70ug\nFITC-labeled geldanamycin (2.5uM): 1x30ul\nHsp90 assay buffer (5X): 1x4ml\nBlack, low binding NUNC microtiter plate: 1 plate\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137281

Size

384Tests

References

1. Kim J, Felts S, Lauger L, He H, Huezo H, Rosen N, and Chiosis G. J. Biomol. Screening 2004; 9(5): 375-381. 2. Howes R, Barril X, Dymock BW, Grant K, Northfield CJ, Robertson AGS, Surgenor A, Wayne J, Wright L, James K, Matthews T, Cheung KM, McDonald E, Workman P, Drysdale MJ. Anal. Biochem. 2006; 350:202-213.