Heat Shock Protein 90, Co-chaperone (HSP90, Telomerase-Binding Protein p23, Progesterone Receptor Complex p23)

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. \nCloned human p23 encodes a protein of 160 aa that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.\n\nApplications:\nSuitable for use in Western Blotting, Immunoprecipitation (use on free or complexed HSP90 Co-chaperone).\n\nRecommended Dilution:\nWestern Blot: 1:1000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nSold under license from Geron

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1834-60

Size

100ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Ch/Bird, GP, Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation.: K.D. Dittmar and W.B. Pratt; J. Biol. Chem. 272, 13047 (1997) Abstract\nHepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids : J.Hu, D. O. Toft and C. Seeger; EMBO Journal 16, 59 (1997) Full Text\n

Additional Info

Recognizes human, mouse, chicken, rabbit and guinea pig HSP90 Co-chaperone. Detects a band of ~23kD by Western blot.