Helicobacter pylori IgG, BioAssay(TM) ELISA Kit

Helicobacter pylori are a spiral bacterium cultured from human gastric mucosa discovered by B.J. Marshall in 1982. Studies have indicated that the presence of H. pylori is associated with a variety of gastrointestinal diseases including gastritis, duodenal and gastric ulcers, non-ulcer dyspepsia, and gastric adenocarcinoma and lymphoma. The studies have also demonstrated that removal of the organism by anti-microbial therapy is correlated with the resolution of symptoms and cure of diseases. An ELISA test of the presence of H. pylori specific IgG antibody is the technique of choice for serologic tests because of its accuracy and simplicity.\n\nSample Type:\nSerum\n\nIntednded Use:\nHelicobacter pylori IgG Test Kit is intended for the quantitative determination of IgG antibodies to Helicobacter pylori in human serum.\n\nTest Principle:\nPurified H. pylori antigen is coated on the surface of microwells. Diluted serum is added to the wells, and the H. pylori IgG-specific antibody, if present, binds to the antigen. All unbound materials are washed away. Enzyme conjugate is added, which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.\n\nKit Components:\nAntigen-Coated Wells 1x96 wells, Microtiter Wells coated with purified H. pylori antigen\nEnzyme Conjugate Reagent 1x13ml, Contains goat anti-human IgG conjugated to horseradish peroxidase with preservatives\nSample Diluent 1x22ml, Contains phosphate buffer with preservatives\nNegative Control 1x150ul/vial, Contains H. pylori IgG negative serum with preservatives\nStandard, 0U/ml, 1x150ul\nStandard, 6.25Uml, 1x150ul\nStandard, 12.5U/ml, 1x150ul\nStandard, 25U/ml, 1x150ul\nStandard, 50U/ml, 1x150ul\nStandard, 100U/ml, 1x150ul \nPositive Control 1x150ul/vial, Contains H. pylori IgG positive serum with preservatives\nWash Buffer Concentrate (20X) 1x50ml, Contains phosphate buffer and Tween 20\nTMB Reagent 1x11ml, Contains 3, 3

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SPECIFICATIONS

Catalog Number

H1840-24H

Size

1Kit

References

1. Marshall, B.J. and J.R. Warren. Unidentified curved bacilli in the stomach of patients with gastritis and Peptic ulceration. Lancet 1: 1311-1314, 1984. 2. Ruaws, E.A.J. and G.N.J. Tytgat. Cure of duodenal ulcer associated with eradication of Helicobacter pylori, Lancet 335: 1233-35, 1990. 3. Perez-Perez, G.I., S.S. Wilkin, M.D. Decker and M.J. Blaswer. Seroprevalence of Helicobacter pylori infection in couples. J. Clin. Microbiol. 29:642-644, 1991.