Hemagglutinin Tag (CYPYDVPDYASL) (HA Tag) (BSA & Azide Free)

A small segment of the viral hemagglutinin coat protein is widely used as an epitope tag to facilitate purification and functional analysis of proteins of interest. Epitope tags are short peptide sequences that are easily recognized by readily available tag-specific antibodies. Ligation of epitope-tag encoding DNA to cloned DNAs of interest produces epitope tagged fusion proteins that can then be subjected to antibody-dependent experimental procedures. Epitope tagging techniques are widely used in the purification, identification, and functional analysis of proteins. Expression vectors for producing tag fusion proteins in a variety of organisms (including bacteria, yeast, insect, and mammalian cells) are commercially available. Some of the more commonly used fusion tags include: c-myc, GFP, GST, HA, His, amd MBP.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested\n\nRecommended Dilutions:\nWestern Blot: 5-10ug/ml for 2 hours at RT.\nImmunoprecipitation: 10ug/mg protein lysate. Use Protein A.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nProteins displaying the YPYDVPDYA HA-tag\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1847-52X

Size

500ul

Applications

IP, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See H1847-52

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Terpe, K., Appl. Microbiol. Biotechnol. 60: 523-533 (2003). 2. Tu, Y., et al., Mol. Biol. Cell 9: 3367-3382 (1998). 3. Bhattacharya and Cabral, Mol. Biol. Cell 15(7): 3123-3131 (2004). 4. Yamanaka, et al., Curr. Biol. 13(9): 734-743 (2003). 5. Chang, et al., Genes Dev. 18(16): 2010-2023 (2004). 6. Munro, S., Pelham, H.R., EMBO J. 3: 3087-3093 (1984). 7. Madeo, F., et al., Mol. Cell 9: 911-917 (2002). 8. Ruckdeschel, K., et al., J. Immunol. 168: 4601-4611 (2002). 9. Field, J., et al., Mol. Cell. Biol. 8: 2159-2165 (1988). 10. Schirmer, E., et al., J. Cell Biol. 153: 479-490 (2001). 11. Bjerling, P., et al., Mol. Cell. Biol. 8: 2170-2181 (2002). 12. Wilson, l.A., et al., Cell 37: 767-78 (1984). 13. Muster, T., et al., J. Virol. 69: 6678-6686 (1995).

Additional Info

Recognizes recombinant proteins containing the Hemagglutinin Tag: YPYDVPDYA.