Hemagglutinin Tag (YPYDVPDYA) (HA Tag)

A small segment of the viral hemagglutinin coat protein is widely used as an epitope tag to facilitate purification and functional analysis of proteins of interest. Epitope tags are short peptide sequences that are easily recognized by readily available tag-specific antibodies. Ligation of epitope-tag encoding DNA to cloned DNAs of interest produces epitope tagged fusion proteins that can then be subjected to antibody-dependent experimental procedures. Epitope tagging techniques are widely used in the purification, identification, and functional analysis of proteins. Expression vectors for producing tag fusion proteins in a variety of organisms\n(including bacteria, yeast, insect and mammalian cells) are commercially available. Some of the more commonly used fusion tags include: c-myc, GFP, GST, HA, His and MBP. \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested\n\nRecommended Dilution:\nWestern Blot: 5-10ug/ml for 2hrs at RT.\nImmunoprecipitation: 10ug/mg protein lysate. Use Protein A.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nProteins displaying the YPYDVPDYA HA-tag\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1847-52

Size

500ul

Applications

IP, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See H1847-52X.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

US Biological application reference: Raikwar, N.S. et al., (2008) Am J Physiol Renal Physiol 295: F1440-F1448. 1. Terpe, K., Appl. Microbiol. Biotechnol. 60: 523-533 (2003). 2. Tu, Y., et al., Mol. Biol. Cell 9: 3367-3382 (1998). 3. Bhattacharya and Cabral, Mol. Biol. Cell 15(7): 3123-3131 (2004). 4. Yamanaka, et al., Curr. Biol. 13(9): 734-743 (2003). 5. Chang, et al., Genes Dev. 18(16): 2010-2023 (2004). 6. Munro, S., Pelham, H.R., EMBO J. 3: 3087-3093 (1984). 7. Madeo, F., et al., Mol. Cell 9: 911-917 (2002). 8. Ruckdeschel, K., et al., J. Immunol. 168: 4601-4611 (2002). 9. Field, J., et al., Mol. Cell. Biol. 8: 2159-2165 (1988). 10. Schirmer, E., et al., J. Cell Biol. 153: 479-490 (2001). 11. Bjerling, P., et al., Mol. Cell. Biol. 8: 2170-2181 (2002). 12. Wilson, l.A., et al., Cell 37: 767-78 (1984). 13. Muster, T., et al., J. Virol. 69: 6678-6686 (1995).

Additional Info

Recognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA. Recognizes the HA-tag fused to either the N- or C-termini of targeted proteins. Species Crossreactivity: A broad species crossreactivity is expected based on conservation of sequence homology.