Heme Oxygenase 1 (HO-1, HSP32, Heat Shock Protein 32)

Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway. Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter, and iron. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs. To date, 3 forms of heme oxygenases \n(HO1-3) have been identified. HO-1 or Hsp-32 (EC 1.14.99.3; mouse/rat 289 aa; human 288 aa, chromosome 22; ~88% homology between the species) is an inducible enzyme. Ho-1 is expressed in most tissues with highest levels in spleen. HO-1 gene expression is inducible by heme, suggesting an important role of HO-1 in heme metabolism. Many other agents or conditions related to oxidant damage such as longer wavelength UV radiation, hyperoxia, hypoxia, hydrogen peroxide, glutathione depletion, endotoxin, and, more recently, nitric oxide (NO) have also been found to stimulate HO-1 expression. HO-1 expression has been shown to increase in benign prostatic hyperplasia (BPH) and malignant prostate tissue.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml. (HO-1 ~31-33kD).\nELISA: 1:50,000. Use \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1847-68M

Size

50ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, NHP

Form

Supplied as a liquid in PBS, 0.1% BSA, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Muller Rm et al (1987) JBC 262, 6795-6802; 2. Shibahara S et al (1985) PNAS 82, 7865-7869; 3. Kageyama H et al (1988) Cancer Res. 48, 4795-4798; 4. Alam J et al (1994) JBC 269, 1001-1009; 5. Keyse SM et al (1989) PNAS 86, 99-103; 6. Yoshida T et al (1988) Eur. J. Biochem. 171, 457-461.

Additional Info

Recognizes human Heme Oxygenase, ~32kD. Does not crossreact with HO-2. Species Crossreactivity: Recognizes a band ~32 kD in monkey, mouse, bovine, rat, and canine.