Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) BioAssay(TM) ELISA Kit (Human)

Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) BioAssay(TM) ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nmпїЅ10nm. The concentration of Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\n\nDetection Range:\n0.156-10ng/ml\n\nSensitivity:\n<0.073ng/ml\n\nSample Matrix:\nTissue homogenates and other biological fluids.\n\nCross Reactivity:\nThis assay has high sensitivity and excellent specificity for detection of Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3). No significant cross-reactivity or interference between Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) and analogues was observed.\n\nStability:\nThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\n\nStorage and Stability:\nDesiccation recommended. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degrees C upon receipt. Store all other reagents at 4 degrees C. Stable for 6 months after receipt of order.

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SPECIFICATIONS

Catalog Number

152649

Size

96Tests

Additional Info

This assay has high sensitivity and excellent specificity for detection of Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3). No significant cross-reactivity or interference between Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) and analogues was observed.