Heparan Sulfate Proteoglycan (HSPG)

The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200kD (aorta) and 30-160kD (kidney) and a core protein size of 24kD or 22kD, respectively, was localized by immunohisto-hemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: < 1ug/ml\nWestern Blot: 1ug/ml\nImmunohistochemistry: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.пїЅ

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SPECIFICATIONS

Catalog Number

H1890-93A

Size

10ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with 10ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Sto?cker G, Meyer HE, Wagener C and Greiling H (1991) Biochem J 274: 415-420 2. Heintz B, Sto?cker G, Rentz U, Melzer H, Mrowka C, Stickeler E, Sieberth HG, Greiling H, Haubeck HD (1995) Hypertension 25: 399-407. 3. Stefanidis I, Heintz B, Sto?cker G, Mrowka C, Sieberth HG, Haubeck HD (1996) J Am Soc Nephrol 7: 1-7. 4. Sto?cker G, Stickeler E, Switalla S, Fischer DC, Greiling H, Haubeck HD (1997) Eur J Clin Chem Clin Biochem 35: 95-99

Additional Info

Recognizes human Heparan-Sulfate-Proteoglycan. Specific for a core protein epitope of a human small basement membrane heparan sulfate proteo-glycan (HS-PG).