Heparan Sulfate Proteoglycan, Large

Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulfate, heparin, chondroitin sulfate, dermatan sulfate or keratan sulfate). Two forms of basement membrane heparan sulfate proteoglycan (HSPG) have been identified. One with a large core protein (> 400kD) and one with a small core protein (30kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:100-1:1000\nImmunohistochemistry (Frozen, paraffin): 1:25-1:200 with avidin-biotinylated horseradish peroxidase complex (ABC) as a detecting reagent.\nOptimum dilutions to be determined by researcher.\n\nHybridoma:\nX63Ag8.653 mouse myeloma cells with spleen cells from a Fisher rat.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1890-87A

Size

100ug

Applications

ICC, IHC, IP, WB

Hosts

Rat

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG2a

References

1.

Additional Info

Recognizes the large Heparan Sulfate Proteoglycan. Reacts with domain IV of the core protein of the large heparan sulfate proteoglycan or perlecan. Reactivity is independent of the galactosaminoglycan moieties. The epitope is therefore not sensitive to heparitinase treatment. Species Crossreactivity: human, mouse, rat, bovine, cod and wolffish