Hepatitis A Virus, P2C, Recombinant (HAV)

Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein and a panel of serum samples from acutely HAV-infected patients. The term "antigenic domain" is used in this study to define a protein region spanned with consecutive overlapping immunoreactive peptides. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1403-1456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1500-1519 aa, includes almost the entire P3B, and the last domain, located at position 1719-1764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. It is interesting to note that four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins.\n\nThe protein contains the HAV core protein P2C immunodominant regions, amino acids: (1211-1234).\n\nSpecificity:\nImmunoreactive with sera of HAV-infected individuals\n\nApplications:\nRecombinant HAV Antigen may be used in ELISA and Western Blot, excellent for detection of HAV with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1900-24A

Size

100ug

Form

Supplied as a liquid in 10mM CBB, pH 9.6, 0.1% SDS, 50% glycerol.

Purity

(same/more than) 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. [Genomic sequence of hepatitis A virus L-A-1 vaccine strain.] Jiang CL, Wang PF, Zhang HY, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2004 Dec;18(4):360-2 2. Interference of hepatitis A virus replication by small interfering RNAs. Kanda T, Kusov Y, Gauss-Mul ler V, Biochem Biophys Res Commun 2004 May 28;318(2):341-5 3. Hepatitis A virus VP3 may activate serum response element associated transcription. Kanda T, Yokosuka O, Imazeki F, Omata M, Scand J Gastroenterol 2003 Mar;38(3):307-13 4. Mutational characteristics in consecutive passage of rapidly replicating variants of hepatitis A virus strain H2 during cell culture adaptation. Hu NZ, Hu YZ, Liu GD, World J Gastroenterol 2002 Oct;8(5):872-8 5. Identification of VP1/2A and 2C as virulence genes of hepatitis A virus and demonstration of genetic instability of 2C. Emerson SU, Huang YK, Brockington A, Purcell RH, J Virol 2002 Sep;76(17):8551-9 6. Adaptation of primate cell-adapted hepatitis A virus strain HM175 to growth in guinea pig cells is independent of mutations in the 5' nontranslated region. Frings W, Dotzauer A, J Gen Virol 2001 Mar;82(Pt 3):597-602.