Hepatitis A Virus, VP3, Recombinant (HAV)

HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.\n\nThe protein contains the HAV VP3 immunodominant regions, amino acids: (304-415).\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1900-46D

Size

100ug

Form

Supplied as a liquid in 10mM CBB, pH 9.6, 0.1% SDS, 50% glycerol.

Purity

~90% pure as determined by 10% PAGE (coomassie staining).

References

1. A novel CD4+ T-helper lymphocyte epitope in the VP3 protein of hepatitis A virus. Sanchez G, Pinto RM, J Med Virol 2004 Apr;72(4):525-32 2. Hepatitis A virus VP3 may activate serum response element associated transcription. Kanda T, Yokosuka O, Imazeki F, Omata M, Scand J Gastroenterol 2003 Mar;38(3):307-13 3. Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design. Haro I, Perez S, Chan WC, FEBS Lett 2003 Apr 10;540(1-3):133-40 4. Membrane fusion induced by a lipopeptidic epitope from VP3 capside protein of hepatitis A virus. Chavez A, Pujol M, Cajal Y, Luminescence 2001 Mar-Apr;16(2):135-43 5. Interaction study of peptide from VP3 capsid protein of hepatitis A virus through monolayers and fluorescence spectroscopy. Sospedra P, Prat J, Mestres C, Luminescence 2001 Mar-Apr;16(2):103-7 6. Membrane fusion by an RGD-containing sequence from the core protein VP3 of hepatitis A virus and the RGA-analogue: implications for viral infection. Chavez A, Pujol M, Alsina MA, Biopolymers 2001 Jan;58(1):63-77.

Additional Info

Immunoreactive with sera of HAV-infected individuals