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Hepatitis B Core Antigen (HBcAg)

Cat no: H1920-10D

Hepatitis B Core Antigen (HBcAg)

Hepatitis B virus is spherical in shape with a diameter of 42nm. It contains a 27nm partially double stranded DNA core enclosed within a lipoprotein coat. The antigenic activity of the nucleocapsid core is designated as hepatitis B core antigen. The antigens in the outer surface are called as hepatitis B virus surface antigens. Core antigens are localized within the nuclei whereas the surface antigens are present in the cytoplasm of the infected cells. Antibodies to surface antigens appear in circulation at an early stage of infection whereas the antibodies to the core antigens are detected after several weeks.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution: \nImmunohistochemistry: (Formalin/paraffin): 1:50-1:100 for 30 min at RT. No special pretreatment is required for staining of formalin/paraffin tissues.\nOptimal dilution to be determined by researcher.\n\nPositive Control: \nHepatitis B virus infected liver\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1920-10D

Size

250ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified antibody fraction from rabbit anti-serum.

References

1. Stahl S, et al. (1982) Proc Natl Acad Sci USA, 79:1606.

Additional Info

Recognizes a protein in the core of hepatitis B virus. Species Crossreactivity: Human.

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