Hepatitis C Virus, E2, Recombinant (HCV)

Full length Recombinant HCV 70kD envelope-GST fusion protein produced in the Baculovirus Expression System.\n\nHepatitis C virus (HCV) is one of the leading causes of chronic liver diseases and B-lymphocyte proliferative disorders, including mixed cryoglobulinemia and B-cell lymphoma. It has been suggested that HCV infects human cells through the interaction of its envelope glycoprotein E2 with a tetraspanin molecule CD81, the putative viral receptor.\n\nRecognizes murine monoclonal antibodies of defined epitope specificity and HCV converted human serum polyclonal antibodies in ELISA and Western Blot. \n\nApplications: \nSuitable for use in ELISA, Western Blot, T-cell activation and Immunization. Other applications have not been tested. \n\nRecommended Dilutions: ELISA: yields titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1920-17F

Size

100ug

Form

Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.

Purity

Purified by glutathione affinity chromatography to (same/more than) 90% purity as determined by SDS-PAGE, reduced.

References

1. Machida. K, et al. (2005) Hepatitis C virus E2-CD81 interaction induces hypermutation of the immunoglobulin gene in B cells. J Virol. 79: 8079-8089. 2. J. Biol. Chem., Vol. 280, Issue 12, 11329-11339, March 25, 2005, Binding of Hepatitis C Virus Envelope Protein E2 to CD81 Up-regulates Matrix Metalloproteinase-2 in Human Hepatic Stellate Cells, Antonio Mazzocca, Silvia Cappadona Sciammetta, Vinicio Carloni, Lorenzo Cosmi, Francesco Annunziato, Takashi Harada