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Hepatitis C Virus NS4, Mosaic, Genotype 2, Recombinant (HCV)

Cat no: H1920-24A1


Supplier: United States Biological
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Artificial mosaic polypeptide composite constructed from diagnostically relevant antigenic regions derived from the NS4 region of different HCV genotypes. The protein contains the fragments of the NS4 immunodominant region: (1691-1710), (1712-1733), (1921-1940). Specificity: Immunoreactive with sera of HCV-infected individuals. Applications: Recombinant HCV NS4 Antigen may be used in ELISA and Western Blots, excellent for detection of HCV with minimal specificity problems. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: H1920-24A1
Size: 100ug
Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 1mM EDTA, 1.5M urea, 50% glycerol.
Purity: (same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
References: 1. Low-level hepatitis C viremia and humoral immune response to NS4 in chronic hepatitis B virus-hepatitis C virus coinfection. Chu CM, Sheen IS, Scand J Gastroenterol 2004 Aug;39(8):778-82 2. [Detection of type specific antibodies to hepatitis C virus NS4 protein in hepatitis C patients by enzyme immunoassay] Kruglov IV, Znoiko OO, Finogenova MP, Voropaev EV, Vopr Virusol 2003 May-Jun;48(3):32-6 3. [Detection of hepatitis C virus-C, E, NS3 and NS4 regions' antigens in hepatocellular carcinoma and liver cirrhosis] Wang L, Li D, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 1998 Dec;12(4):326-9 4. Characterization of monoclonal antibodies and epitope mapping of the NS4 protein of hepatitis C virus. Masalova OV, Lakina EI, Atanadze SN, Ulanova TI, Immunol Lett 2002 Oct 1;83(3):187-96 5. [Gene chimeric fusion and expression of nucleocapsid NS3 regions and NS4 regions of hepatitis C virus genome] Shen XC, Li M, Sheng Wu Gong Cheng Xue Bao 2001 Jan;17(1):46-9 6. Prospective reevaluation of risk factors in mother-to-child transmission of hepatitis C virus: high virus load, vaginal delivery, and negative anti-NS4 antibody. Okamoto M, Nagata I, Kaji S, Tazawa Y, J Infect Dis 2000 Nov;182(5):1511-4.

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