Hepatocyte Growth Factor, Recombinant, Human (HGF, HGFB, DFNB39, F-TCF, Hepatopoeitin-A, HPTA, Scatter Factor, SF)

Hepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cella and sinusoidal endothelial cells in the liver and pancreas.\n\nSource:\nDNA sequence encoding the complete human Hepatocyte Growth Factor protein isoform 1 (comprising the signal peptide, alpha and beta chains) was expressed in modified human 293 cells.\n\nBiological. Activity:\nThe ED50 of HGF is typically 20-40 ng/ml as measured in a cell proliferation assay using the human HepG2 cell line.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Under reducing conditions, the HGF protein migrates as a broad band between ~80 and 110kD in SDS-PAGE due to post-translational modifications, in particular glycosylation. The unmodified HGF has a predicted monomeric molecular mass of 79.7kD. The protein separates into a number of isoforms with a pI between 6 and 9 in 2D PAGE. The unmodified HGF protein has a predicted pI of 8.2. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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